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By Alexander S. Raikhel

Progress in Mosquito Research presents readers with the newest interdisciplinary experiences at the subject. it truly is an important reference resource for invertebrate physiologists, neurobiologists, entomologists, zoologists, and bug chemists, with quantity fifty one concentrating on fresh growth in mosquito research.

  • Presents a finished evaluate of contemporary growth in mosquito science
  • Written by means of leaders of their respective components of mosquito research
  • Ideal source for invertebrate physiologists, neurobiologists, entomologists, zoologists, and bug chemists

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A. 95 (10), 5505–5510. , 2015. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat. Biotechnol. 33 (2), 187–197. , 2001. Transcriptional gene silencing in plants: targets, inducers and regulators. Trends Genet. 17 (1), 29–35. , 2012. Genome-wide manipulations of Drosophila melanogaster with transposons, Flp recombinase, and PhiC31 integrase. Methods Mol. Biol. 859, 203–228. , 2015. Tools for Anopheles gambiae transgenesis. G3 (Bethesda) 5 (6), 1151–1163. , 2014.

Nat. Methods 2 (8), 583–585. , 2011. piggyBac transposon remobilization and enhancer detection in Anopheles mosquitoes. Proc. Natl. Acad. Sci. A. 108 (39), 16339–16344. , 2012. Gal4based enhancer-trapping in the malaria mosquito Anopheles stephensi. G3 (Bethesda) 2 (11), 1305–1315. , 2015. Understanding the DNA damage response in order to achieve desired gene editing outcomes in mosquitoes. Chromosome Res. 23 (1), 31–42. , 2013. Post-integration silencing of piggyBac transposable elements in Aedes aegypti.

Regardless of the sex chromosome karyotype, what appears to be in common in all mosquitoes is the presence of an M-factor at the top of the sex determination hierarchy that initiates male development. In Anopheles culifacies, it was found that individuals with the XXY genotype developed as males (Baker and Sakai, 1979), suggesting the presence of a dominant M-factor on the Y chromosome. This is in contrast to D. melanogaster where there is no M-factor and the XXY genotype develops as females due to having two X chromosomes and therefore maintaining the X:A ratio (Bridges, 1921; Cline, 1993; Marin and Baker, 1998).

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