Download Insect Histology: Practical Laboratory Techniques by Pedro Barbosa PDF

By Pedro Barbosa

This name is a miles wanted replace of Barbosa's self-published Manual of simple suggestions in Insect Histology. it's a laboratory guide of 'traditional' and 'modern' insect histology innovations, thoroughly revised utilizing state-of-the-art technique performed at the present time and contains new immunohistochemical innovations now not formerly appeared at.

Insect Histology is designed as a source for pupil researchers, in academia and undefined, who require uncomplicated details at the systems which are crucial for the histological demonstrate of the tissues of bugs and similar organisms.

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Additional resources for Insect Histology: Practical Laboratory Techniques

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Although rarely appropriate for intact insects or larvae, individual organs can be dissected and snap frozen in liquid nitrogen or in a dry ice/isopentane bath. These fresh frozen tissues can be cryosectioned for downstream applications including but not limited to immunohistochemistry, RNA or DNA isolation, microarray analysis, laser capture microscopy, and proteomic and metabolomic analyses. 2 Aldehyde based fixatives Application: Recommended as a fixative which causes no shrinkage to certain insect tissues.

Transfer to iodine alcohol to remove excess mercuric chloride. 5 Movat’s FSA [39] Application: Recommended as a fixative that will give consistently reproducible staining reactions in all insect tissue. Recommended as useful for staining with hematoxylin-eosin and Wismar’s quadrachrome. Formula: Formalin Mercuric Chloride Glacial Acetic Acid Distilled Water 20 pt 4 pt 5 pt 80 pt Procedure: 1. Fix 15 mm tissue blocks; should be fixed for a maximum of 24 hr. 2. Rinse quickly in a 15% iodine alcohol solution (70%).

Note: 1. Store in non-metallic containers (indefinitely if necessary). 6 Petrunkevitch’s [40] Formula: Mercuric Chloride Nitric Acid Glacial Acetic Acid Absolute Alcohol Distilled Water 5 pt 5 pt 45 pt 100 pt 150 pt Procedure: 1. Fix for 30 min (for small specimen) or up to 6 hr (for larger specimens). 2. Wash for a few hours in running water. 3. Transfer to several changes of iodine alcohol (70%). 7 Schaudin’s [30] Formula: Mercuric Chloride (saturated aqueous) Alcohol (95%) Glacial Acetic Acid (immediately before use) 66 pt 33 pt 5 pt Note: 1.

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