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Initial spikes were Pu(IV), and equilibrium time was 7 days. 45 pm. *lo00 molecular weight (Amicon UM2). to changes in the concentration of solids present, with the reduced form being relatively more abundant (in solution) a t low solids concentrations. Although not conclusive, this trend is the same as that previously seen for seawater. Water samples from the open ocean, in which particle concentrations are very low, showed a higher percentage of dissolved Pu present in the reduced forms than did samples collected near shore.
In both solutions, reduced Pu solubilities may have been related to association with the cells. In the P U ~ ( D T P A )solutions, ~ an additional complication may have arisen from the partial dissociation of Pu from the DTPA a t lower pH values. Investigations were, therefore, undertaken to determine whether the decrease in solubility was the result o f 1. Transport of Pu across the fungal membrane at low pH. 2. Binding of Pu to the membrane surface. 3. Formation of insoluble hydrolysis products.
0 *Calculations were based on the parameter values given in Table 8 and the ambient concentrations of COC given in Table 7. NELSON. LARSEN, AND PENROSE 42 matter), natural COC compounds also are important in the redox behavior of Pu. We have observed a general trend both in field measurements and laboratory experiments for oxidized Pu to be present as a sizeable component only when COC concentrations are low. This effect was suggested in Table 5 and is more clearly illustrated in Fig. 6. In that figure, the data from the ANL pond experiment shown in Fig.