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By Justin A. Daniels

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2007). , 2007). g. how do populations vary with seasonality Current and Emerging Microbiology Issues of Potable Water in Developed Countries 31 and from one country to another? , 2006). Better education and increased awareness by the general public and pool operators could potentially reduce the number and impact of swimming pool and other recreational waterrelated outbreaks (Robertson et al. 2002a). Improved detection methods, with the ability to differentiate species, can be useful in the assessment of infection and identification of contamination sources.

2007). , 2007). , 1999). More accurate Microsporidial epidemiological data is urgently required to accurately assess the importance of this emerging pathogen. EMERGING AND OPPORTUNISTIC PATHOGENS Opportunistic microbes are a subset of the emerging pathogens and include species of both fecal and environmental origin. , 2004). , 2004). , 2004). Examples of opportunistic bacterial pathogens of potable water origin include Aeromonas hydrophila, L. pneumophila, M. avium, and Pseudomonas aeruginosa.

Real-time PCR (qPCR) is more rapid as it monitors the amount of PCR products as they are being produced and does not require size separation (Kubista et al. 2006; Zhang and Fang, 2006). In addition qPCR is more sensitive than traditional PCR methods and can estimate the initial concentration of nucleic acids. As with standard PCR methods, multiplexing qPCR is limited by the difficulty in optimizing reactions so that all targets are amplified with similar efficiencies. Moreover, the number of assays that can be multiplexed is limited by the number of fluorescent dyes that can be distinguished in a single reaction (typically no more than 4, depending on the specifications of the instrument being used).

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